Precision-engineered botanical matrices, enzymatic aids, and high-potency concentrates manufactured under certified GMP and ISO protocols, fully harmonized with EU and Croatian food safety directives.
Standardized macamides and glucosinolates for vitality and endurance formulas; water-soluble matrix ideal for clean-label adaptogenic blends.
Crisp natural acidity profile designed to blend seamlessly with Reb-M stevia glycosides, eliminating metallic sweetness in citrus beverages.
Carrier-optimized spray-dried powder preserving volatile terpene aromatics; acts as a natural bulk carrier in reduced-sugar bakery glazes.
Abundant in Omega-7 palmitoleic acid and flavonoids, serving functional yogurt drinks and anti-aging wellness supplements across Central Europe.
Native L-ascorbic acid content delivering organic antioxidant stabilization; synergistic partner with clean stevia blends in isotonic beverages.
High-activity biological catalysts driving fructose conversion and enzymatic glucosyl-stevioside transglycosylation for rounded sweetness.
Cleavers extract standardized for iridoids and polyphenols, answering growing demand for herbal detox bitters and botanical tonic preparations.
Standardized to cordycepin and beta-glucans; perfect for nootropic energy shots and recovery sticks, flavored cleanly with high-grade Reb-D.
Precision selection of diterpene glycoside isolates derived from Stevia rebaudiana Bertoni leaves, comparing molecular structures, sweetness indices, and gustatory kinetic curves.
Analytical metrics evaluated against European Commission Regulation (EU) No 231/2012, Regulation (EU) 2021/1156 (E960c), and pharmacopeial purity protocols.
| Compound / Matrix Name | CAS Registry | Molecular Formula | Sweetness (vs Sucrose) | Sensoric Onset & Lingering | Regulatory Status (EU) |
|---|---|---|---|---|---|
| Rebaudioside M (Reb-M) | 1220616-44-3 | C₅₆H₉₀O₃₃ | 300× – 350× | Immediate onset, zero bitterness, pure sucrose profile | Approved (E960a / E960c) |
| Rebaudioside D (Reb-D) | 63279-13-0 | C₅₀H₈₀O₂₈ | 250× – 300× | Fast onset, negligible licorice notes, superior mouthfeel | Approved (E960a) |
| Rebaudioside A 98% (Reb-A) | 58543-16-1 | C₄₄H₇₀O₂₃ | 200× – 240× | High intensity, mild astringency at >500 ppm concentrations | Approved (E960a) |
| Enzymatic Stevia (Glucosyl Stevioside) | 865314-10-9 | Variable Polyglucosyl | 120× – 180× | Completely eliminates bitter aftertaste, rounded lingering | Flavouring w/ Modifying Properties |
| Total Steviol Glycosides (STG 95%) | 57817-89-7 | Composite Mix | 200× – 250× | Cost-effective baseline formulation, slight licorice baseline | Approved (E960a) |
The fundamental challenge of natural steviol glycosides lies in their interaction with the human bitter taste receptors hTAS2R4 and hTAS2R38. The basic steviol core possesses an aglycone diterpene backbone that triggers bitterness if the C-13 and C-19 ester and ether bonds do not carry a sufficient load of sugar moieties.
In standard stevioside, the presence of only three glucose units leaves the hydrophobic binding sites exposed. By contrast, Rebaudioside M carries six beta-D-glucosyl units, arranged symmetrically on the C-13 and C-19 positions. This steric hindrance physically blocks access to the binding pockets of bitter taste receptors, resulting in an exceptionally clean, sucrose-identical temporal profile that activates only the sweet heterodimer hT1R2/hT1R3.
For food scientists in Croatia developing low-glycemic products, Torzi Biotech provides precision isomer separation via cross-flow nanofiltration and multi-column macroporous adsorption resin systems, guaranteeing consistent Reb-M and Reb-D ratios from batch to batch.
Column: Amino-bonded phase (NH₂), 5 μm, 4.6 × 250 mm
Mobile Phase: Acetonitrile : Water (80:20 v/v), isocratic or gradient elution
Flow Rate: 1.0 mL/min | Column Temp: 30°C ± 1°C
Total Ash: ≤ 1.0% | Heavy Metals (Pb, As, Cd, Hg): ≤ 1 ppm each
Residual Solvents (Ethanol, Methanol): In full compliance with Directive 2009/32/EC (Ethanol ≤ 5,000 ppm, Methanol ≤ 200 ppm).
Croatia's strategic position along the Adriatic tourism corridor and its modern retail landscape (Zagreb, Split, Rijeka, Osijek) demand versatile sugar-reduction formulas tailored to domestic and export demands.
In response to Croatia's expanding wellness tourism and coastal summer hospitality, non-alcoholic botanicals, isotonic spritzers, and carbonated craft ciders are replacing high-fructose corn syrups with Torzi Stevia Reb-M 95%. Combined with our Spray-Dried Orange or Grapefruit Juice Powders, the formulation achieves vibrant fruit flavor with zero lingering aftertaste, maintaining crystal clarity without sedimentation even under long UV exposure on sunny Dalmatian terraces.
Croatia has a long-standing culinary heritage in fermented dairy beverages and high-protein quark cheeses. Lowering sugar in probiotic yogurts often disturbs the delicate acid-sweet balance. Glucosyl-steviosides and Reb-D operate synergistically with native lactic acids, preserving the smooth rounded mouthfeel without triggering lactic cultures or mutating fermentation kinematics, enabling dairy brands in Slavonia and Central Croatia to achieve Nutri-Score A rankings.
Industrial bakeries and chocolate manufacturers facing EU-mandated sugar reduction quotas require intense thermal stability. Torzi Biotech's steviol glycosides remain thermally robust at temperatures up to 200°C without undergoing caramelization degradation or Maillard-induced off-flavors. When compounded with erythritol, allulose, or prebiotic inulin, they provide the missing bulk volume for traditional Croatian pastries, filled biscuits, and chocolate bars.
Essential guidance on importing, formulating, and distributing stevia-based products in accordance with the Croatian Ministry of Health (Ministarstvo zdravstva) and EFSA frameworks.
The Croatian Institute of Public Health (HZJZ) actively promotes voluntary and mandatory sugar reduction across staple groceries. Steviol glycosides provide immediate reformulatory pathways without losing shelf-appeal.
Products incorporating steviol glycosides must declare "with sweetener" or "sweetened with steviol glycosides from stevia" under Regulation (EU) No 1169/2011, establishing high consumer trust on supermarket shelves.
Sports fitness culture in Zagreb and coastal sports clubs has driven high demand for zero-sugar pre-workouts. Combining stevia with active amino acids like Taurine and Creatine Monohydrate ensures clean flavor delivery.
Direct maritime container shipping from China to the Port of Rijeka (Luka Rijeka) and air cargo into Zagreb (Franjo Tuđman Airport) ensures predictable lead times, minimal demurrage, and direct customs transit.
Whether you require pilot testing samples (1 kg - 5 kg) or full-scale multi-metric ton container shipments delivered directly to your plant in Croatia, our technical R&D team and export managers are ready to support your formulation specifications.
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Shaanxi Torzi Biotech Co., Ltd. operates from the world-renowned botanical processing corridor in Xi'an, China. With over 23 years of specialized extraction history, our facility covers 12,447 square meters of controlled industrial infrastructure, audited on-site by SGS Group.
Every step of extraction, purification, and packaging is recorded within an unbroken digital audit trail, ensuring complete lot-specific validation for EU customs authorities.
Raw stevia leaves undergo species authenticity testing via TLC and HPLC. Samples are quarantined until certified free from heavy metals (Pb ≤ 1.0 mg/kg, Cd ≤ 1.0 mg/kg, As ≤ 1.0 mg/kg, Hg ≤ 0.1 mg/kg) and mycotoxins before entry into water extraction tanks.
Using food-grade polymeric macroporous resins, the crude water extract is desorbed with food-grade aqueous ethanol. Specific fractions (Reb-A, Reb-D, Reb-M, and Stevioside) are eluted based on distinct polarity, achieving target purity benchmarks above 95% to 99%.
Purified solutions pass through sub-micron sterile filtration before low-temperature spray drying. Materials are hermetically vacuum-sealed inside Class 100,000 cleanrooms using double-walled pharmaceutical polyethylene liners in corrugated fiber drums.
Critical commercial and logistical considerations for R&D managers, regulatory executives, and procurement officers importing botanical ingredients into Croatia and the broader European Single Market.
Our Stevia Leaf Extracts are categorized under food additive number E 960a (Steviol glycosides from Stevia) or E 960c (Enzymatically produced steviol glycosides), fully compliant with European Commission Regulation (EU) No 231/2012 and subsequent amendments. For applications requiring flavor classification, we also provide FEMA GRAS enzyme-treated stevia modifiers.
We routinely supply goods under Incoterms 2020 including FOB Shanghai/Qingdao, CIF Luka Rijeka, and DAP/DDP Zagreb. Air courier sample shipments (via DHL/FedEx) arrive within 4–6 business days for immediate lab trial runs, while FCL/LCL ocean freight into Rijeka typically takes 28–35 days from vessel departure.
Every commercial invoice is backed by a batch-specific Certificate of Analysis (COA) containing precise HPLC assay percentages, an allergen declaration (Regulation (EU) No 1169/2011), GMO-free statement, heavy metals certificate, microbiological test sheet, and a non-irradiation statement. Phytosanitary certificates and Certificate of Origin (COO) are supplied as standard.
Yes. Many Croatian bakery and beverage manufacturers request custom pre-mixes combining high-intensity Reb-M or Reb-D with soluble dietary fibers (polydextrose, resistant dextrin), erythritol, or our natural fruit powders (e.g., spray-dried orange or grapefruit juice powders). We provide dry-blended homogenates with uniform particle distribution down to 100 mesh.
For research and product pilot trials, our standard MOQ starts from 1 kg for high-purity single isomers such as Rebaudioside M 95% and Rebaudioside D 95%. Commercial production runs for standardized total steviol glycosides (SG95 / Reb-A 97%) start at 25 kg fiber drums, with volume tiered discounts extending to 20-foot container volumes.
In accordance with rigorous EU food safety alerts, our Xi'an testing facility analyzes every batch via LC-MS/MS for ethylene oxide and 2-chloroethanol with a limit of quantification (LOQ) of ≤ 0.02 mg/kg. Furthermore, raw materials are pre-screened for hazardous pyrrolizidine alkaloids (PA) per EU Regulation 2020/2040.
Collaborate with an engineering-first botanical manufacturer. Access our analytical laboratory data, complete technical dossier, and comprehensive sample evaluation packs customized to your formulation goals.
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