Pharma Grade
Hyaluronic Acid Powder | Food & Cosmetic Grade
High & Low Molecular Weight fractions (3 kDa to 2.2 MDa), glucuronic acid titer ≥45%, non-animal bio-fermentation origin.
Portfolio Architecture
Featured Standardized Extracts & Bio-Nutritional Precursors
Supplying pharmaceutical and functional nutrition laboratories in Split, Zagreb, and across the Adriatic with tested, validated bulk raw materials featuring HPLC-confirmed active fraction titers.
100% Pure
Marine Fish Collagen Oligopeptide Powder
Enzymatic hydrolysis, mean MW <1,000 Da, 90%+ protein content, instant cold-water solubilization with zero marine odor.
Naringin Flavonoids
Freeze-Dried Grapefruit Juice Concentrate Powder
Cold vacuum freeze-dried, retaining bioflavonoid glycosides, natural naringin complexes, and native vitamin co-factors.
Citrus Bioflavonoids
Spray-Dried Orange Juice Concentrate Powder
Low-temperature spray atomization, high retention of hesperidin fractions and citrus flavanones, instant beverage reconstitution.
MCT Rich
Organic Coconut Milk & Coconut Water Powder
Cold-pressed micro-encapsulated medium chain triglycerides, native potassium electrolyte balance, vegan carrier matrix.
Standardized 30%
Chaga Mushroom Extract Powder (Inonotus obliquus)
Dual-extracted wild sclerotium, standardized to ≥30% chromogenic beta-glucans and triterpenoid betulinic acid fractions.
Dual Extracted
Reishi Mushroom Extract Powder (Ganoderma lucidum)
Log-cultivated red fruiting body, 30%–50% polysaccharides with HPLC-calibrated ganoderic acids for immunomodulatory formulations.
Flavonoid Rich
Galium Aparine (Cleavers) Botanical Extract
Concentrated iridoid glycoside and polyphenolic polyphenol profile, pharmaceutical excipient compatibility, food grade certified.
Technical Whitepaper
Phytochemical Fractionation & The Kinetics of High-Titer Flavonoids
Flavonoids represent one of the most structurally diverse classes of secondary plant metabolites. Modern nutraceutical and pharmaceutical engineering demands precise chromatographic isolation of these benzo-γ-pyrone derivatives to yield reproducible bioactivity.
Molecular Speciation and Titer Control
Flavonoids possess a characteristic C6-C3-C6 carbon skeleton consisting of two aromatic rings linked through a three-carbon heterocyclic ring. In commercial dietary supplements and dermaceuticals, maintaining the native configuration—whether as free aglycones (such as Quercetin, Apigenin, and Luteolin) or as O- and C-glycosides (such as Rutin, Hesperidin, and Isoquercitrin)—dictates intestinal permeability, cellular bioavailability, and resistance to thermal degradation.
Our extraction methodologies rely on multi-stage macroporous resin adsorption chromatography (utilizing food-grade styrenic polymers) to fractionate polar polyphenols away from non-functional cellulose, heavy pectins, and trace sugars. This achieves purities exceeding 95% to 98% by HPLC, minimizing excipient load during tablet compression or high-speed hard capsule filling.
Key Analytical Assay Metrics
- Chromatographic Profiling: Reversed-Phase HPLC-DAD against certified USP and Ph. Eur. primary reference materials.
- Isomeric Separation: Complete resolution of Rutin vs. Isoquercitrin and Naringin vs. Hesperidin isomers.
- Residual Solvent Elimination: Aqueous and pharmaceutical ethanol extractions conforming to ICH Q3C Class 3 limits.
- Submicron Particle Engineering: Jet-milled micronization providing 80–200 mesh particle distribution curves for enhanced dissolution rates.
"For modern formulators operating under European Union regulatory frameworks, crude botanical powders are no longer viable. Active therapeutic efficacy, shelf-life kinetic stability, and claim substantiation depend entirely on guaranteed chromatographic assay numbers and validated polyphenol-to-flavonoid ratios."
| Botanical Actives | Dominant Flavonoid Fraction | Standardized Specification | Testing Methodology | Primary Application Target |
|---|---|---|---|---|
| Sophora Japonica L. | Rutin / Quercetin / Troxerutin | ≥95.0% - 98.0% Flavones | HPLC / UV-Vis Spectrophotometry | Vascular Integrity & Venous Insufficiency |
| Citrus Aurantium L. | Diosmin / Hesperidin / Naringin | 90% Diosmin / 10% Hesperidin (MPFF) | Reversed-Phase HPLC-DAD | Microvascular Elasticity & Anti-Edema |
| Camellia Sinensis L. | Epigallocatechin Gallate (EGCG) | ≥50.0% - 98.0% Polyphenols | HPLC-MS Grade Validation | Metabolic Optimization & Cellular Protection |
| Scutellaria Baicalensis | Baicalin / Baicalein | ≥85.0% - 90.0% Baicalin | HPLC Gradient Elution | Neuro-Calmative & Dermato-Protective |
Regulatory Governance
CE Certification, Croatian HALMED Guidance & EU Compliance
Navigating the stringent landscape of European health claims, heavy metal restrictions, and pesticide ceilings requires an accountability-driven primary manufacturer with comprehensive traceability.
Regulation (EC) No 396/2005
Full compliance with European Union pesticide maximum residue levels (MRLs). Every extract lot is screened via multi-residue GC-MS/MS and LC-MS/MS across 450+ controlled agricultural compounds, safeguarding client brands against market withdrawals.
Regulation (EU) 2023/915
Heavy metal thresholds strictly controlled via Atomic Absorption Spectrometry (AAS) and Atomic Fluorescence Spectrometry (AFS). Lead (Pb) <3.0 ppm, Cadmium (Cd) <1.0 ppm, Mercury (Hg) <0.1 ppm, and Arsenic (As) <1.0 ppm are validated on every production batch.
Dossier & Technical Backing
We supply comprehensive technical packages including full Batch COA, Flow Charts, Non-GMO Statements, Allergen Declarations, BSE/TSE-Free Certificates, Residual Solvent Statements (ICH Q3C), and HALAL/KOSHER accreditations for simplified Croatian Ministry filings.
Local Ecosystem Integration
Industrial Application Scenarios in Split & the Adriatic Basin
Split serves as the pharmaceutical, biotechnical, and marine-nutraceutical gateway of Dalmatia. From specialized contract manufacturers in Dugopolje industrial zones to advanced cosmeceutical developers in Split city, our flavonoid extracts meet targeted local manufacturing needs.
Microvascular & Venous Health Formulations
Dalmatia's demographic and Mediterranean lifestyle markets focus heavily on circulatory wellness. Micronized Purified Flavonoid Fractions (Diosmin 90% and Hesperidin 10%) are the gold-standard raw materials for regional Croatian laboratories developing OTC venotonic supplements and heavy-leg relieving tablets.
Marine-Botanical Hybrid Nutricosmetics
Split's position on the Adriatic coast has spurred innovation in hybrid anti-aging solutions. Formulators combine our Marine Fish Collagen Oligopeptides and Hyaluronic Acid with potent polyphenol-rich botanical flavonoids to formulate skin elasticity boosters that target UV-induced oxidative stress.
Mediterranean Functional Beverage Fortification
Regional beverage producers are enhancing fruit juices, sports drinks, and herbal tonics with water-dispersible citrus bioflavonoid complexes. Our spray-dried citrus and polyphenol powders dissolve without sediment, imparting functional antioxidant benefits while maintaining clarity.
Enterprise Manufacturing Capabilities
Torzi Biotech: Certified Infrastructure & Technical Rigor
Headquartered in Xi'an near the Qinling botanical corridor, Shaanxi Torzi Biotech Co., Ltd. operates as a vertically integrated extraction manufacturer rather than an intermediary broker. SGS verified with 23+ years of technical operational legacy.
Full Batch Traceability Under One Roof
With an expansive 12,447 m² modern facility and 89 specialized personnel, Torzi Biotech executes eight tightly monitored production phases: rigorous raw material botanical selection, counter-current extraction, high-efficiency vacuum concentration, macroporous resin adsorption, spray drying in pressurized HEPA-filtered cleanrooms, mechanical milling, homogenous micro-blending, and vacuum aseptic packaging.
Our in-house analytical laboratories are equipped with twin Agilent High-Performance Liquid Chromatographs (HPLC), Gas Chromatographs (GC-MS) for solvent residues, and Atomic Absorption Spectrophotometers (AAS) for micro-contaminant detection. This eliminates testing bottlenecks and guarantees that analytical certificates match every kilogram dispatched to European distribution centers.
Multi-Stage Extraction Battery
Cleanroom Spray Tower
HPLC Assay & Purity Isolation
Heavy Metal AAS Validation
Strategic Sourcing Insights
Market Growth & Logistics Corridors to Split-Dalmatia
Croatian wellness demand is undergoing an evolution from generic plant powders to high-potency bio-fractionated concentrates. Understanding trade channels and port dynamics ensures uninterrupted seasonal manufacturing.
Port of Split & Rijeka Multimodal Logistics
Our European logistics architecture supports direct maritime container transfers into the Port of Split (Luka Split) or northern transit via Port of Rijeka. Consignments are backed by air express options (Zagreb/Split airports) for pilot trials and urgent formulation ramp-ups.
Clean-Label & Non-GMO Trends in Croatia
Consumers in Dalmatia are demanding transparency regarding excipients, silicon dioxide, and artificial binders. Our flavonoid extracts are processed without synthetic carriers, utilizing native rice-derived or organic maltodextrin aids only when specifically commissioned by client formulators.
Private Label & Custom Contract Blending
In addition to pure active bulk drums, we support Split-based brand incubators with OEM turnkey solutions: hard-shell vegetarian capsules (HPMC), custom stick-pack sachets, and precision formulation blends ready for retail branding with full EU compliant labeling.
Procurement Due Diligence
Split Procurement FAQ: Technical, Regulatory & Commercial
Common inquiries addressed to our technical sales and regulatory affairs divisions by Croatian QA directors, purchasing officers, and lab heads.
How do you verify the distinction between total polyphenols and pure flavonoids?
We do not rely solely on nonspecific colorimetric assays like Folin-Ciocalteu, which can be confounded by non-flavonoid reducing sugars. We execute high-resolution Reversed-Phase HPLC-DAD against pure reference standards (USP/Ph. Eur.), isolating individual target molecules (e.g., Quercetin aglycone, Rutin trihydrate, or Naringin) with distinct retention times.
What is the standard lead time for delivery into Split or Zagreb warehouses?
For pre-stocked pharmaceutical actives and standardized mushroom extracts, air cargo delivery to Split (SPU) or Zagreb (ZAG) takes approximately 5 to 8 business days post-clearance. For bulk ocean consignments via Port of Split or Rijeka, transit schedules range from 28 to 35 days, including complete Croatian Customs (Carinska uprava) import documentation.
Are your extraction facilities inspected by third-party international auditors?
Yes. Shaanxi Torzi Biotech Co., Ltd. has undergone exhaustive on-site supplier verification and facility audits conducted by SGS Group. Our operational floor plans, cleanrooms, and sanitation procedures conform to ISO 9001 and ISO 22000 quality management certifications, backed by international KOSHER and HALAL accreditations.
What are your Minimum Order Quantities (MOQs) for pilot formulation trials?
To support Croatian R&D teams and academic research at institutions like the University of Split, we supply evaluation sample batches and maintain low pilot-scale MOQs starting at 1 kg for high-purity active monomers. Commercial production orders for standardized bulk drums typically range from 25 kg to multi-ton consignments.
Can you provide micronized powder grades for high-speed tableting operations?
Yes. We specialize in custom particle size distributions. Using pharmaceutical fluid-energy jet mills, we engineer powders down to D90 < 20 μm (600–800 mesh) or maintain uniform 80–120 mesh granules. This guarantees laminar flowability during encapsulation and prevents segregation in high-speed rotary tablet presses.
What stability data and packaging safeguards protect hygroscopic extracts?
All bulk powders are sealed in double medical-grade polyethylene food liners inside hermetic, tamper-evident aluminum-foil bags or fiber drums with silica moisture-scavengers. Nitrogen flushing is deployed for easily oxidizable polyphenol compounds. Each delivery includes accelerated and real-time stability dossier summaries.
Partner with an Accountable Primary Extraction Manufacturer
Contact our senior technical sales team to request analytical sample kits, verified batch Certificates of Analysis, or detailed commercial price schedules tailored for delivery to Split, Dalmatia, and across the EU.